Scutellaria barbarum identification:
Identification of traits: 15-40 cm in length, hairless or hairy on the flower axis. The roots are slender. The stem has a quadrangular shape with a dark purple or brownish green surface. Leaves opposite, shortly sessile or subsessile; leaf blade sessate or sessate, sessately sately sately sately lanceolate or lanceolate, 1.5------------- Wedge shape, whole or a few with obvious blunt teeth, dark green on top, light green on the bottom; crisp and brittle.
Inflorescences are born at the branches, corollas 2-lipped, brownish yellow or dark blue-purple, ca. 1.2 cm long, coats, but corollas often detached from the product, leaving a spoon-shaped squat and a helmet-like captain. Contains 4 spheroidal nutlets, light brown. The whole grass is soft and easy to break. Weiqi, bitter taste. It is better to use green and bitter taste.
Microscopic identification:
Leaf surface preparation: The epidermal cells are long and polygonal, the vertical wall is wavy, and the upper epithelial cells are large, 55-93 μm long and 14-40 μm wide. The epidermal cells are 25-48 μm long and 11-25 μm wide. Non-glandular hair 1-2 cells, conical, wall with ridges, basal cells with radial stratum corneum texture; near the leaf margin non-glandular hair, composed of 1-3 cells, 48-141μm long. The glandular scale has fewer upper surfaces and more lower surface. The glandular head is spherical and oblate, consisting of 7-8 cells, 50-75 μm in diameter, and single cells in the gland; glandular hair is present in the epidermis, and 4 cells are present. The glandular head is composed of about 28 μm in diameter and a single cell of the glandular stem. The stomata are present in the lower epidermis, straight-axis.
Physical and chemical identification:
1. Take 10 grams of this product powder, add 50 ml of 80% ethanol, reflux on a water bath for half an hour, filter it through hot water, and filter the following test:
2. Take 1 ml of the filtrate, add a little magnesium powder and 4 drops of concentrated hydrochloric acid, and the solution is gradually reddish. (check flavonoids)
3. Take 1 ml of the filtrate and add 1-2 drops of 1% ferric chloride test solution. The solution is dark green. (check phenols)
4. The above remaining filtrate was evaporated to dryness on a water bath, and the residue was dissolved in 5 ml of 5% hydrochloric acid and filtered. Add about 1 ml of the filtrate to each of the three test tubes, and add 1-2 drops of cesium iodide test solution, potassium iodide test solution and tungstic acid test solution, respectively, to produce a precipitate. (check alkaloids)
5, thin layer chromatography sample preparation: the product powder (20 mesh) 2 grams, add 10 ml of ether, reflux for half an hour, decanted diethyl ether, the residue was added methanol 15 ml, reflux for half an hour, cooled, filtered, filtrate for point Sample use. The amount of sample is 10 ml. Adsorbent: silica gel H (Huangyan Fluorescence Chemical Plant), 0.5% CMC plated, activated at 110 ° C for half an hour. Developer: Toluene-ethyl formate-formic acid (3:3:1). Spreading distance 12cm. Spots were observed under a UV lamp (365 nm).
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